Figure 4

Protein expression analysis of IMR-90 fibroblasts following irradiation. Western blot analysis of un-irradiated control (CTL), bystander (BYS) and irradiated (IRR) cells comparing (a) phosphorylated-AKT, total AKT, phosphorylated-GSK3β, GSK3β and actin and (b) phosphorylated β-catenin, β-catenin and actin up to 8 hours. (c) Densitometric analyses were performed on representative immunoblots, bands were background corrected, normalized to actin and sham-irradiated controls. The relative levels of phosphorylated AKT, phosphorylated GSK3β and phosphorylated β-catenin are shown as fold change compared with controls. The thin black line represents time-matched controls.