Screening of STSs across the Y chromosome. Diagrammatic representation summarizing STS analysis in the representative samples for screening of recombinations in the MSY region of the Y chromosome. We used 37 STSs to screen for different recombination deletions including P5-Proximal P1, P5-Distal P1, gr/gr, b1/b3, b2/b3, TSPY-TSPY besides checking for the presence of AZFa region. sY14 located in SRY gene was used as positive control. The sample IDs are given on the left side while the STSs analyzed are on top. Continuous blue line is indicative of intactness of the STSs screened while the interrupting orange bars reflect absence of the same.