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Table 2 Production of chemokines by lung lymphocytes ex vivo after immunization with Ad85A i.d. or i.n..

From: Chemokine gene expression in lung CD8 T cells correlates with protective immunity in mice immunized intra-nasally with Adenovirus-85A

 

Absorbance at 450nm (± SD)

Chemokine

i.d.

i.n.

CCL2

0.89 (± 0.427)

1.368 (± 0.131)

CCL3

0.334 (± 0.066)

0.745 (± 0.008)

CCL4

0.278 (± 0.011)

0.760 (± 0.028)

CCL5

0.386 (± 0.098)

1.470 (± 0.211)

CXCL9

0.259 (± 0.059)

0.566 (± 0.034)

 

Conc (pg/ml)

CXCL16

3.34 (± 0.339)

6.095 (± 0.977)

  1. Lung lymphocytes were isolated from mice immunized 3 weeks previously and cultured in RPMI+10% FCS for 6 hours at 37°C without antigen stimulation. The supernatants were assayed by ELISA for the indicated chemokines. The table shows the mean absorbance at 450 nm (± standard deviation (SD)) of 6 mice per group. For CXCL16, the mean concentration of CXCL16 (pg/ml, ± SD) of 6 mice per group is shown.