Figure 8
Down-regulation of RelB expression by HDAC inhibitor TSA. Primary B cells from healthy donor (CD19+), cells from male or female CLL patients (CLLM and CLLF, respectively), or Ramos B cell line (established from Burkitt lymphoma's patient), used as control, were untreated (NT) or preincubated for 16 hr with 200 nM trichostatin A (TSA). Total protein fraction was prepared as described in Methods section. Western blot analyses show that TSA induced RelB down-regulation in all B cell types tested.