Figure 5

Gene expression assessed in Lep ^ob/ob mice by quantitative real-time RT-PCR. The real-time RT-PCR data were calculated using the relative quantification method by comparison with the expression of the housekeeping gene CypB (Pfaffl, 2001). The boxes represent the medians and interquartile ranges. Comparisons of the groups were made with the Mann-Whitney test. Control n = 4; Lep^ob/ob n = 4. *P < 0.01.