Expression of TICs and their component genes. (A-F) Each panel contains expression data for a TIC and its component genes, and is labeled with the splice distance. The leftmost plot in each panel shows the expression of the TIC splice using qRT-PCR measurements relative to GAPDH in prostate tumor samples T1-T3, matched normal prostate samples N1-N3, and a commercial sample of normal prostate (C). Error bars indicate the standard error over 2 replicate measurements. The rightmost plots in each panel show expression of the 5' and 3' genes for the T1-T3 and N1-N3 samples, as measured by RNA-Seq in reads per kilobase per million total reads (RPKM). TICs are presented from panel A to panel F in order of increasing TIC splice expression. For panels A-C, expression of the 5' and 3' genes are plotted on the same scale. For panels D-F, because expression of the 3' gene is extremely low relative to that of the 5' gene, expression of each 3' gene is plotted on its own scale. Panel F for MSMB-NCOA4 has the greatest variance of expression values across samples and shows that TIC splice expression correlates with 5' gene expression, but not 3' gene expression. (G) Relationship between TIC and 5' gene expression, shown as a scatterplot. (H) TIC splicing efficiency, computed as TIC splice expression divided by the 5' gene expression, for each sample. In panels G and H, plot symbols A-F correspond to the TICs labeled in panels A-F.