Figure 7
Distant fusions. (A) Expression level of TMPRSS2-ERG e1-e4 and e1-e5 fusion splices in prostate tumor and normal samples measured by qRT-PCR, compared with ERG expression as measured by RNA-Seq. qRT-PCR measurements are shown for prostate tumor samples T1-T3, matched normal prostate samples N1-N3, and a commercial sample of normal prostate. RNA-Seq measurements are shown fir T1-T3 and N1-N3. (B) Comparison of SEC31A-C6orf62 expression level with downstream C6orf62 expression. Fusion is observed only in the T3 sample. (C) Comparison of IRS2-NUFIP1 expression level with downstream NUFIP1 expression. Fusion is observed only in the T2 sample. (D) CGH microarray data for chromosome 21, containing the TMPRSS2-ERG fusion. A corresponding genomic deletion is observed in the T3 sample, but not in T2, indicating that the gene fusion in T2 is due to translocation. (E) CGH microarray data for chromosome 13, containing the IRS2-NUFIP1 fusion. No corresponding genomic deletions are observed.