Skip to main content
Figure 4 | BMC Medical Genomics

Figure 4

From: The use of ultra-dense array CGH analysis for the discovery of micro-copy number alterations and gene fusions in the cancer genome

Figure 4

Array CGH using 1 M platform and whole genome amplified DNA reveals artifacts due to whole genome amplification. Array CGH of whole-genome amplified DNA from the MCF-7 cell line compared to non-amplified DNA, with magnification of two small segments in chromosomes 1 and 2. (A, B) Data obtained with 1 M platform. In three independent experiments (top 3 sections) DNA was amplified using the Phi29 polymerase kit. The fourth experiment (bottom section) was performed without WGA. Arrows indicate some of the WGA artifacts. (C, D) Array CGH results from the 1 M platform shown as overlaid moving averages obtained in 4 experiments; 3 with WGA-DNA (blue, green, red) and 1 without amplification (purple). (C) Zoom-out on chromosome 1. (D) Zoom-out on chromosome 2. (E, F) Data obtained with 244 K platform. In three independent experiments (top 3 sections) DNA was amplified using Phi29 polymerase kit. The fourth experiment (bottom section) was performed without WGA. Note the small "wave" effects are seen only in the 1 M arrays when using WGA.

Back to article page