2D-electrophoresis and multiplex immunoassay proteomic analysis of different body fluids and cellular components reveal known and novel markers for extended fasting

Table 2 Protein identifications by MS/MS analysis of biofluid and blood cell proteins which were significantly increased or decreased upon extended fasting (36 h), as compared with overnight fasting (12 h)

Sample type	Spot	Accession #	Protein identification	Fold- change	p-value	Adjusted p-value	Mascot score	Sequence Coverage %	Matched peptides
Plasma	1	P06727	Apolipoprotein A-IV (ApoA4)	-1.80	<0.001	<0.001	426	69	34
	2	P00738	Haptoglobin B chain	-1.15	<0.001	0.009	87	32	8
	3	P00738	Haptoglobin B chain fragment	-1.14	<0.001	0.006	139	33	17
	4	P36955	Pigment epithelium-derived factor	-1.09	<0.001	0.061	113	35	16
	5	P02741	C reactive protein (CRP)	1.42	<0.001	0.061	74	17	6
PBMC	6	P52566	Rho GDP-dissociation inhibitor 2 (RhoGDI2)	-2.78	<0.001	<0.001	63	23	4
	7	O00299	Chloride intracellular channel protein 1 (CLIC1)	5.30	<0.001	<0.001	73	23	4
Saliva	8	P01833	Polymeric-immunoglobulin receptor (PIGR)	-3.16	<0.001	0.030	161	15	10
Urine	9	P02768	Serum albumin precursor	1.83	0.004	0.185	162	45	22
	10	P02768	Serum albumin precursor	2.02	0.001	0.056	152	32	16

ISSN: 1755-8794