2D-electrophoresis and multiplex immunoassay proteomic analysis of different body fluids and cellular components reveal known and novel markers for extended fasting

Table 3 Plasma proteins of which absolute levels significantly changed due to extended fasting (36 h) compared with overnight fasting (12 h), as assessed using the multiplex immunoassay (RBM)

Protein	Accession #	Fold-change	p-value	Adjusted p-value
IGF-1	P01343	-2.72	<0.001	<0.001
Leptin	P41159	-2.59	<0.001	<0.001
Erythropoietin	P01588	-1.56	0.080	0.187
IgE	n.a.	-1.54	0.002	0.013
IL-8	P10145	-1.51	<0.001	0.001
MMP-3	P08254	-1.48	<0.001	<0.001
Thyroid Stimulating Hormone	P01222	-1.47	<0.001	<0.001
Insulin	P01308	-1.29	<0.001	0.001
Carcinoembryonic Antigen	P06731	-1.29	0.037	0.116
IL-1beta	P01584	-1.19	<0.001	<0.001
PAI-1	P05121	-1.19	0.002	0.013
Apolipoprotein A-1 (ApoA1)	P02647	-1.18	0.006	0.025
MCP-1	P13500	-1.17	<0.001	0.001
Eotaxin	P51671	-1.16	0.001	0.005
VEGF	P15692	-1.14	<0.001	<0.001
IL-16	Q14005	-1.13	<0.001	0.002
EGF	P01133	-1.12	0.085	0.190
IL-18	Q14116	-1.12	0.013	0.048
Alpha-Fetoprotein	P02771	-1.12	<0.001	<0.001
Fatty Acid Binding Protein	P05413	-1.07	0.006	0.025
IgA	n.a.	-1.07	0.043	0.116
Factor VII (F7)	P08709	-1.06	0.061	0.157
Creatine Kinase-MB	P06732	-1.04	0.075	0.181
IL-10	P22301	-1.04	0.041	0.116
Glutathione S-Transferase	P09211	-1.04	0.010	0.041
Tissue Factor (TF)	P13726	-1.03	0.004	0.019
Prostatic Acid Phosphatase	P15309	-1.03	0.018	0.067
Alpha-2 Macroglobulin (A2M)	P01023	1.02	0.021	0.072
Complement 3	P01024	1.02	0.028	0.092
TIMP-1	P01033	1.02	0.005	0.025
IL-12p70	P29459	1.03	0.038	0.116
VCAM-1	P19320	1.05	0.011	0.044
Serum Amyloid P (SAP)	P02743	1.07	0.072	0.179
Lipoprotein (a)	P08519	1.09	0.041	0.116
MIP-1beta	P13236	1.09	0.048	0.127
TNF-alpha	P01375	1.10	0.042	0.116
EN-RAGE	P80511	1.17	0.085	0.190
Ferritin	P02794	1.27	<0.001	0.001
C Reactive Protein (CRP)	P02741	1.55	<0.001	0.002

ISSN: 1755-8794