Figure 5

qRT-PCR validation on splice variants differentialy expressed in stage 1- and stage 4+ tumors. Quantitative RT-PCR was performed for three spliced genes including PKM2 (NM_002654 vs. NM_182470 & NM_182471), KIF1B (NM_015074 vs. NM_183416) and MAP2 (NM_001039538 vs. NM_002374) in 5 stage 1- and 5 stage 4+ tumors. Differential expression of splice variants was evaluated by calculating expression fold changes between splice variants of the spliced gene in each sample, which were further centered by median of values obtained in stage 1- and stage 4+ tumors. The expression of splice variants is significantly different between stage 1- and stage 4+ tumors for all three tested spliced genes.