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Table 1 Comparison of the duration of the DAE analysis between the HR-1ℱ and the LightScanner¼ instruments, for 96 samples

From: Detecting differential allelic expression using high-resolution melting curve analysis: application to the breast cancer susceptibility gene CHEK2

DAE step

HR-1ℱ instrument

LightScannerÂź instrument

PCRs

Same duration

Same duration

Data acquisition

2 days

12 minutes

Data analysis

1 full day

15 minutes

  1. The HR-1ℱ instrument can only analyze a single sample per run making data analysis time consuming. The LightScanner¼ instrument, with its 384-well plate format, is of greater practical efficiency. Data analysis was performed using an analysis tool that we developed.