Knowledge-based functional and network analyses of human homologs of zebrafish estrogen-responsive genes. A. Selected top molecular and cellular functions significantly (P-value < 0.05) associated human homologs of zebrafish estrogen-responsive genes. Histograms are read with reference to 'Percentage of Human Homologs Used in Analysis' axis while solid and dashed lines are read with reference to '-Log P-value' axis. 'Percentage of Human Homologs Used in Analysis' refers to the percentage of the total 289 human homologs used in the analysis. Solid line represents the inverse logarithm (base 10) of the p-value for each group of biological association [greater -Log (P-value) correlates with greater statistical significance] while the dashed-line represents the significant threshold where the p-value = 0.05. B. Top most significant network assembled de novo using 30 focus molecules associated with cell cycle, cell death and reproductive system development and function. The inset shows the molecules in the network that are involved in more than one function. C. Second top significant network assembled de novo from 30 focus molecules associated with DNA replication, recombination and repair, cell cycle and cancer. Red nodes represent genes encoding respective molecules are up-regulated in response to estrogen and green nodes represent down-regulation. Interactions are represented by edges connecting 2 nodes. Different types of edges represent the direction and effect of interactions as given in the legends.