Knowledge-based signalling pathway and network analyses of conserved estrogen-responsive genes between zebrafish and human cancer cell lines. A. Selected top signaling pathways significantly (P-value < 0.05) associated with conserved estrogen responsive genes. Histograms are read with reference to 'Percentage of Signalling Pathway' axis while solid and dashed lines are read with reference to '-Log P-value' axis as in Figure 3A. 'Percentage of Signalling Pathway' refers to the percentage of the total molecules in each respective signalling pathway. B. Top most significant network assembled de novo using 27 focus molecules associated with selected enriched signalling pathways (mitotic roles of polo-like kinase, AHR signalling, cell cycle regulation by BTG family proteins, cell cycle: G2/M DNA damage checkpoint regulation). The inset shows molecules associated with the selected canonical pathways. C. Top significant signalling pathway, mitotic roles of polo-ike kinase, showed that the conserved estrogen-responsive genes encoding the respective molecules were all up-regulated (red nodes) from mitotic entry through transition from metaphase to anaphase and mitotic exit followed by cytokinesis and subsequent centrosome separation. Legends are similar to Figure 3B and 3C.