Clustering analysis of the expression ratio of well-known genes (sense transcripts) and their corresponding AFAS transcripts showing altered expression balances. Clustering analysis was conducted for expression ratios obtained by the random priming approach. Expression ratios obtained by the oligo-dT priming approach were aligned to the clustering results of the random priming approach. The green color indicates higher expression detected by the sense probe compared with the corresponding AFAS probe (3-fold of more). The red color indicates lower expression detected by the sense probe compared with its antisense counterpart (1/3-fold of less). Sense-AFAS probe pairs in the figure correspond to those listed in Table 1. (a) Clustering result for sense-AFAS pairs where the sense gene is up-regulated and the corresponding AFAS gene is down-regulated in cancer tissues. (b) Clustering result for sense-AFAS pairs where sense gene is down-regulated and the corresponding antisense gene is up-regulated in cancer tissues.