MiRNome analysis of umbilical cord MSCs and differentiated neural precursors. (A) (upper and middle) Histograms showing the induction of neural differentiation in WJ-MSCs. WJ-MSCs were subjected into neurogenesis, and total RNAs were collected at the indicated time points for real-time PCR validation. neurofilament (NF), neurogenesis-related genes; nestin, stemness gene. (lower) Immunofluorescence assays on NeuN protein. Day 3 neuronal precursors derived from WJ-MSC were used. (B) A heat map shows the miRNA expression patterns in WJ-MSCs (0d) and derived neural precursors which were differentiated for 9 days (9d). MiRNAs in red, increased expression; in blue, decreased.