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Table 1 Validation by qRT-PCR of FSHD dysregulation of gene expression

From: Gene expression during normal and FSHD myogenesis

  Microarray profiling   qRT-PCR    
Gene FSH/Ctl
FCb
p-value FSH/Ctl
FCb
p-value No. of
FSH
samples
No. of Ctl
samples
Myoblasts       
PCOLCE a 5.9 4E-05 3.5 0.04 5 6
RUVBL2 2.2 5E-04 1.7 0.01 7 8
TGFB2 -2.8 4E-04 -1.9 0.02 9 8
EIF2C2 -1.6 0.007 -1.6 0.03 8 8
Myotubes       
BGN 11.0 < 1E-06 16.8 0.04 8 9
DCN 6.4 6E-04 7.5 0.01 8 9
PCOLCE 10.2 < 1E-06 3.7 0.03 6 7
MYOM1 -8.9 2E-06 -2.9 0.05 4 4
SFRS9 3.5 < 1E-06 3.5 0.01 6 6
CAPG 3.0 0.002 3.2 0.02 8 9
IGFBP6 2.5 0.02 3.5 0.01 8 9
FRAS1 -3.02 0.001 -2.2 0.001 4 4
  1. aFull gene names and primer-pairs are given in Additional File 5 TableS4
  2. bFC, fold change in RNA signal was determined by exon microarray analysis or qRT-PCR