Table 1 Validation by qRT-PCR of FSHD dysregulation of gene expression
| Â | Microarray profiling | Â | qRT-PCR | Â | Â | Â |
|---|---|---|---|---|---|---|
Gene | FSH/Ctl FCb | p-value | FSH/Ctl FCb | p-value | No. of FSH samples | No. of Ctl samples |
Myoblasts | Â | Â | Â | Â | Â | Â |
PCOLCE a | 5.9 | 4E-05 | 3.5 | 0.04 | 5 | 6 |
RUVBL2 | 2.2 | 5E-04 | 1.7 | 0.01 | 7 | 8 |
TGFB2 | -2.8 | 4E-04 | -1.9 | 0.02 | 9 | 8 |
EIF2C2 | -1.6 | 0.007 | -1.6 | 0.03 | 8 | 8 |
Myotubes | Â | Â | Â | Â | Â | Â |
BGN | 11.0 | < 1E-06 | 16.8 | 0.04 | 8 | 9 |
DCN | 6.4 | 6E-04 | 7.5 | 0.01 | 8 | 9 |
PCOLCE | 10.2 | < 1E-06 | 3.7 | 0.03 | 6 | 7 |
MYOM1 | -8.9 | 2E-06 | -2.9 | 0.05 | 4 | 4 |
SFRS9 | 3.5 | < 1E-06 | 3.5 | 0.01 | 6 | 6 |
CAPG | 3.0 | 0.002 | 3.2 | 0.02 | 8 | 9 |
IGFBP6 | 2.5 | 0.02 | 3.5 | 0.01 | 8 | 9 |
FRAS1 | -3.02 | 0.001 | -2.2 | 0.001 | 4 | 4 |