Targeted high throughput sequencing in clinical cancer Settings: formaldehyde fixed-paraffin embedded (FFPE) tumor tissues, input amount and tumor heterogeneity

Table 1 Sequencing statistics

ID	sample specification	Target size	# uniquely aligned reads	% reads on target	# of enriched regions	# of SNVs called (20×)
Pat1_B	Snap-frozen	52 Mb	66, 114, 467	75.4%	200, 175	18, 287
Pat1_B	FFPE	52 Mb	71, 590, 872	74.7%	200, 032	17, 810
Pat11_B	Snap-frozen	3.9 Mb	28, 043, 981	62.8%	12, 274	3, 000
Pat11_T	Snap-frozen	3.9 Mb	18, 302, 565	71.3%	12, 226	2, 511
Pat11_B	FFPE	3.9 Mb	9, 311, 629	67.1%	12, 175	2, 577
Pat11_T	FFPE	3.9 Mb	15, 928, 525	73.7%	12, 254	2, 883
Pat2_T	500 ng	3.9 Mb	8, 760, 773	81.1%	12, 220	2, 652
Pat2_T	1500 ng	3.9 Mb	9, 686, 320	79.9%	12, 243	2, 848
Pat2_T	3000 ng	3.9 Mb	6, 810, 410	80.4%	12, 204	2, 602
Pat3_B	Benign tissue	3.9 Mb	19, 617, 926	67.7%	12, 122	2, 002
Pat3_T	Focus-1	3.9 Mb	28, 798, 280	68.9%	12, 227	2, 328
Pat3_T	Focus-2	3.9 Mb	31, 939, 154	69.6%	11, 142	2, 513
Pat4_B	Benign tissue	3.9 Mb	8, 878, 742	66.2%	12, 281	2, 662
Pat4_T	Focus-1	3.9 Mb	8, 768, 332	64.2%	12, 288	2, 645
Pat4_T	Focus-2	3.9 Mb	9, 178, 790	65.3%	12, 269	2, 706
Pat5_B	Benign tissue	3.9 Mb	25, 957, 461	63.2%	12, 274	2, 646
Pat5_T	Focus-1	3.9 Mb	65, 372, 578	64.4%	12, 261	2, 388
Pat5_T	Focus-2	3.9 Mb	25, 957, 461	63.0%	11, 157	2, 342

ID: Identification number of the patient's tissue with B for benign tissue and T for tumor tissue.

ISSN: 1755-8794