Covariance matrix showing correlation of PC1 with disease recurrence. (a) PC1, despite being developed with unsupervised approaches, appeared to correlate well with EMT, disease recurrence, disease progression, and differentiation status, but not with gene signatures linked to adenoma vs. carcinoma, MSI status, or mucinous vs. nonmucinous cancers. Moreover, PC1 appeared to be anti-correlated with RAS, MYC, Proliferation, and colon laterality. PC1 distinguishes good and poor prognosis patients in the MCC data set (b) as well as in two independent test sets: (c) Netherlands Cancer Institute (NKI, #13) (L.V.V.) and (d) Lin et al data sets .