DNA methylation differences at growth related genes correlate with birth weight: a molecular signature linked to developmental origins of adult disease?

Table 1 Correlation of mechanism-based candidate gene expression levels with birth weight

Gene Symbol	Transcript ID	HumanHT-12 v3 Expression vs. Birth Weight % (R²)		Real Time RT-PCR Expression vs. Birth Weight % (R²)
		Cord Blood (n = 48)	Placenta (n = 48)	Cord Blood	Placenta
IGF1	ILMN_2056087	2.0E-04	0.017	nd	nd
	ILMN_1709613	0.003	0.002
IGF1R	ILMN_1675048	0.009	0.045	nd	nd
IGF2	ILMN_1699867	1.3E-05	0.004	1.2E-04 (n = 190)	4.5E-04 (n = 254)
	ILMN_2298035	0.008	0.001
	ILMN_2413956	0.003	0.003
IGF2BP1	ILMN_1733807	0.007	1.0E-04	nd	nd
IGF2BP2	ILMN_1702447	0.003	0.016	0.022 (n = 119)	1.0E-07 (n = 114)
IGF2BP3	ILMN_1807423	0.056	0.007	nd	nd
IGF2R	ILMN_1807662	0.006	3.0E-04	0.005 (n = 194)	6.1E-04 (n = 241)
IGFBP1	ILMN_2387385	0.014	0.001	ne	0.052 (n = 150)
	ILMN_1728445	0.001	0.001
IGFBP2	ILMN_1725193	0.006	0.031	ne	0.003 (n = 110)
IGFBP3	ILMN_1746085	0.007	0.002	ne	0.001 (n = 135)
	ILMN_2396875	0.009	0.002
IGFBP4	ILMN_1665865	0.006	0.003	nd	nd
IGFBP5	ILMN_2132982	0.014	0.002	nd	nd
	ILMN_1750324	0.001	0.003
IGFBP6	ILMN_1669362	0.001	0.009	nd	nd
IGFBP7	ILMN_2062468	2.5E-05	0.005	nd	nd
INS	ILMN_1666966	0.022	0.034	nd	nd
INSR	ILMN_1670918	0.058	0.031	nd	nd
INSRR	ILMN_1715374	0.007	3.9E-05	nd	nd
PHLDA2	ILMN_1671557	0.036	0.001	nd	nd
PLAGL1	ILMN_1815121	0.001	0.009	0.006 (n = 105)	0.013 (n = 136)
	ILMN_2356955	0.014	0.004
IGF2/IGF2R*		n/a	n/a	0.002 (n = 186)	0.002 (n = 241)

Multiple entries represent data for multiple transcripts on the array. The best correlation obtained in each group is shown in bold
* Ratio IGF2/IGF2R expression
n/a = not applicable
nd = not done
ne = not expressed

ISSN: 1755-8794