Bar chart showing relative NGAL, granulysin and resistin expression in survivors and non-survivors. A total of 176 samples were analysed using RT PCR. The ++ Ct method was used to calculate normalised data . There was over-expression of NGAL and resistin and under-expression of granulysin in cases compared to controls (data not shown). Relative gene expression of NGAL granulysin and resistin were increased in non-survivors compared to survivors Error bars = mean+/−2SE NS = not significant.