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Table 4 Primer sequences and probe numbers for each assay

From: Novel biomarker combination improves the diagnosis of serious bacterial infections in Malawian children

Gene

Forward Primer Sequence

Reverse Primer Sequence

Universal Probe Library

GNLY

AGGGTGACCTGTTGACCAAA

CAGCATTGGAAACACTTCTCTG

17

LCN2

TCACCTCCGTCCTGTTTAGG

AGGTAACTCGTTAATCCAGGGTAA

61

RETN

TGCAGGATGAAAGCTCTCTG

CATGGAGCACAGGGTCTTG

45

ACTB

ATTGGCAATGAGCGGTTC

GGATGCCACAGGACTCCAT

11

B2M

TTCTGGCCTGGAGGCTATC

TCAGGAAATTTGACTTTCCATTC

42

GAPDH

AGCCACATCGCTCAGACAC

GCCCAATACGACCAAATCC

60

GNB2L1

GCTACTACCCCGCAGTTCC

CAGTTTCCACATGATGATGGTC

55

HMBS

AGCTATGAAGGATGGGCAAC

TTGTATGCTATCTGAGCCGTCTA

25

HPRT1

TGACCTTGATTTATTTTGCATACC

CGAGCAAGACGTTCAGTCCT

73

PGK-1

CTGTGGCTTCTGGCATACCT

CTTGCTGCTTTCAGGACCA

42

RPL13A

GAGGCCCCTACCACTTCC

TGTGGGGCAGCATACCTC

28

RPL32

GAAGTTCCTGGTCCACAACG

GCGATCTCGGCACAGTAAG

17

SDHA

AGAAGCCCTTTGAGGAGCA

CGATTACGGGTCTATATTCCAGA

69

TBP

GCTGGCCCATAGTGATCTTT

CTTCACACGCCAAGAAACAGT

3

YWHAZ

CGTTACTTGGCTGAGGTTGC

TGCTTGTTGTGACTGATCGAC

9

  1. 12 endogenous transcripts (ACTB, B2M, GAPDH, GNB2L1, HMBS, HPRT1, PGK-1, RPL13A, RPL32, SDHA, TBP and YWHAZ) were screened and analysed using the GeNORM algorithm [28].
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BMC Medical Genomics

ISSN: 1755-8794

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