Figure 6

Real time RT-PCR Quantitation of ANGPTL7, LAMA5 and SHROOM2 in CT-, PF-, and DC-derived cells. Differential expression of (a) ANGPTL7, (b) LAMA5 and (c) SHROOM2 was directly confirmed by qRT-PCR in CT-, PF- and DC-derived fibroblasts. Values are mean ± SEM of two independent experiments performed in triplicate. GAPDH was used as an internal control. Statistical analyses were performed by Student's t test. Relative quantification of gene expression was calculated by comparing δ Ct values between CT-, PF- and DC- derived fibroblasts. p < 0.05 was considered significant