Figure 3

Flow cytometry analysis with OVCAR3, SKOV3, and TOV-112D were performed before and after anchorage-independent culture at least three times. CD133 ⁺ and CD44 ⁺ populations were enriched in a sphere culture system in TOV112D and OVCAR3 cells; however, CD44 ⁺ populations were initially high in the SKOV3 cell line.