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Table 2 Validation of miRNA microarray results using quantitative RT-PCR: miRNAs differentially expressed between OVCAR3 cells from the CD133+ sphere-forming subpopulation compared with cells cultured in a conventional adherent environment

From: MicroRNA profiling of a CD133+spheroid-forming subpopulation of the OVCAR3 human ovarian cancer cell line

 

MicroRNA microarray

qRT-PCR

 

Fold change

Pvalue

Fold change (mean ± SD)

has-miR-205

11.78

0.001

11.76 ± 1.32

has-miR-146a

7.33

<0.001

5.19 ± 4.14

has-miR-200a

2.45

0.0265

2.21 ± 2.54

has-miR-200b

2.13

0.014

1.81 ± 0.54

has-miR-31

2.35

0.024

2.45 ± 1.59

has-miR-1202

0.41

0.013

0.58 ± 0.22

has-miR-1181

0.47

0.023

0.67 ± 0.28

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BMC Medical Genomics

ISSN: 1755-8794

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