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Table 2 Validation of miRNA microarray results using quantitative RT-PCR: miRNAs differentially expressed between OVCAR3 cells from the CD133+ sphere-forming subpopulation compared with cells cultured in a conventional adherent environment

From: MicroRNA profiling of a CD133+spheroid-forming subpopulation of the OVCAR3 human ovarian cancer cell line

  MicroRNA microarray qRT-PCR
  Fold change Pvalue Fold change (mean ± SD)
has-miR-205 11.78 0.001 11.76 ± 1.32
has-miR-146a 7.33 <0.001 5.19 ± 4.14
has-miR-200a 2.45 0.0265 2.21 ± 2.54
has-miR-200b 2.13 0.014 1.81 ± 0.54
has-miR-31 2.35 0.024 2.45 ± 1.59
has-miR-1202 0.41 0.013 0.58 ± 0.22
has-miR-1181 0.47 0.023 0.67 ± 0.28