Validation of microarray results by real-time qRT-PCR in an independent set of samples. A. Eight miRNAs identified as significantly different between AAA and controls in the microarray study were evaluated by qRT-PCR in an independent set of twelve AAA and seven control samples. B. Five down regulated miRNAs validated by qRT-PCR (from panel A), were analyzed using thirteen additional samples from elective AAA repairs (eAAA) and eleven samples from ruptured AAAs (rAAA). The expression levels for each miRNA were adjusted to the expression levels of U6. The p values were calculated using the Wilcoxon rank-sum test. Significance between each comparison is shown. NS, not significant; *, p < 0.05; **, p < 0.001; ***, p < 0.0001.