Figure 3

A network of miRNAs miR-133a, miR-133b, miR-331-3p, miR-204, and their target genes. miR-30c-2* was not included because it is a miRNA* strand that is usually degraded upon miRNA loading to the RISC complex [17]. Bioinformatic analysis predicted 222 genes (see Additional file 4: Table S4) with upregulated expression in AAA based on a prior microarray study [9] were targets of miR-133a, miR-133b, miR-331, or miR-204. The predicted minimum free energy of the miRNA and target mRNA hybridization from RNAhybrid is shown by the line and node color. Black lines indicate that the 3'UTR sequence was not available from Biomart, and the minimum free energy was not calculated. The figure was generated using CytoScape® [20].