Table 1 Primer pairs used for direct bisulfite sequencing and combined bisulfite restriction analysis of hypermethylated genes
Gene | GenBank Accession No. |  | Primer (5' → 3') |
|---|---|---|---|
PITX2 | NM_000325.5 | F | TAGTGATAGGCGTTTCGGGTT |
| Â | Â | R | CCACTACATACTAACAAACACTCAAAT |
TLX3 | NM_021025 | F | TCGGTTGAGGATTAGAGGGATT |
| Â | Â | R | AACGCCACCTAACCATCTATTC |
OTX2 | NM_172337.1 | F | AGTTGTGTTAGGTTGAGGGAG |
| Â | Â | R | AATCCCAAAAACCTTTTTAAA |
MNX1 | NM_001165255 | F | TTTAAGAAATAGCGAGAGGGAG |
| Â | Â | R | AAACGCTCGTAACATAATCCC |
NR2F2 | NM_001145155 | F | CGTATCGTGGATTTGGAGTAGGGTATT |
| Â | Â | R | AACAAACTCGCTAACAAATAAACRACATT |
TFAP2C | NM_003222 | F | CGAAGTGTTAGGGTTTTGTGTGT |
| Â | Â | R | CGACCTTAAACAACAACCAAATCC |