Table 1 Primer pairs used for direct bisulfite sequencing and combined bisulfite restriction analysis of hypermethylated genes
| Gene | GenBank Accession No. | Primer (5' → 3') | |
|---|---|---|---|
| PITX2 | NM_000325.5 | F | TAGTGATAGGCGTTTCGGGTT |
| R | CCACTACATACTAACAAACACTCAAAT | ||
| TLX3 | NM_021025 | F | TCGGTTGAGGATTAGAGGGATT |
| R | AACGCCACCTAACCATCTATTC | ||
| OTX2 | NM_172337.1 | F | AGTTGTGTTAGGTTGAGGGAG |
| R | AATCCCAAAAACCTTTTTAAA | ||
| MNX1 | NM_001165255 | F | TTTAAGAAATAGCGAGAGGGAG |
| R | AAACGCTCGTAACATAATCCC | ||
| NR2F2 | NM_001145155 | F | CGTATCGTGGATTTGGAGTAGGGTATT |
| R | AACAAACTCGCTAACAAATAAACRACATT | ||
| TFAP2C | NM_003222 | F | CGAAGTGTTAGGGTTTTGTGTGT |
| R | CGACCTTAAACAACAACCAAATCC |