Figure 2

miRNAs miR-7 and miR-128 down-regulate EGFR in HEY cells. (A &B) Histograms showing the average of independent biological repicates of qPCR determined EGFR RNA levels following transfection of miR-7 or miR-NC (A), and miR-128 or miR-NC (B) in HEY cells using GAPDH as the endogenous control. Statistical analysis for qPCR was carried out using randomization (REST 2008 software) [42] with at least 1000 iterations [Note that the REST Software determines gene 95% confidence intervals for gene expression ratios using a bootstrapping technique without normality or symmetrical distribution assumptions. Thus, the error bars will not necessarily be equal in the plus and minus directions] (*** p <0.001, ** p <0.05); (C &D ) Representative western blots of EGFR protein levels after transfection of miR-7 and miR-NC (C) or miR-128 and miR-NC (D); (E &F) Histograms showing average densitometric analyses of independent biological replicates of immunoblots measuring protein levels of EGFR following transfection of miR-7 and miR-NC (E) or, miR-128 and miR-NC (F). Statistical analysis of immunoblots was carried out using a 2-tailed t-test (** p <0.01).