Figure 4From: The effects of MicroRNA transfections on global patterns of gene expression in ovarian cancer cells are functionally coordinated Examples of how miRNA regulation of hub genes indirectly affect the expression of large numbers of downstream genes. (A) Among genes differentially expressed after miR-7 transfection, RELA/NF-κB (circled in red) acts as a hub gene, and is found to target many of the differentially expressed genes. One of these, IL1-beta (circled in red) is significantly down-regulated and targets additional differentially expressed genes, thus, amplifying the effect of NF-κB down-regulation. (B) Caveolin-1 (circled in red) is a significantly down-regulated hub gene and a predicted target of miR-128. One of CAV1’s many direct downstream target genes is SMAD2 (also circled in red; regulation shown by arrow/edge pointing from Caveolin-1 to SMAD2), which itself acts as another hub gene. Several downstream targets of SMAD2 are also differentially expressed following miR-128 transfection. Thus by targeting the hub gene CAV1, miR-128 can regulate the non-target hub gene SMAD2 and trigger a ripple effect on the expression of down-stream genes. Key: blue filled circle - significantly down-regulated gene as determined by microarray analysis; red filled circle – significantly up-regulated gene as determined by microarray analysis; green edges: established activating interaction; red edges: established inhibitory interaction; grey edges: predicted interaction of unknown significance.Back to article page