2-DE analysis of differentially expressed protein spots between HCT116 gankyrin
and wild-type cells. Total cell lysates (125 μg) of each cell lines were subjected to 2-DE analysis and detected by silver staining. The pH gradient of the first-dimension electrophoresis (3~10, 3~5.6, 6~11) is shown on top of the gels, and the migration of molecular mass markers for SDS-PAGE in 2-D is shown on the left side. The original gel size was 20 × 20 × 0.1 cm3. The marked protein spots were significantly altered between HCT116 gankyrin−/− and wild-type cells. The results of identification by mass spectrometry are listed in Table 1.