CAFs derived from Her2+ breast cancer significantly enhances the migration of T47D cells in vitro. In vitro transwell assays comparing T47D migration in the absence (orange) or presence of CAFs isolated from ER (black), Her2 (blue) and TNBC (green) primary human breast cancer tumors were performed. Each experiment was performed in duplicates using CAFs derived from at least two different patients. One CAF cell line of each subtype was tested in 2 independent experiments (open vs. closed circles). The second CAF cell line of each subtype (squares) was tested in duplicate in one independent experiment, for a total of 6 tests. Lines show mean ± SEM.