Table 4 Primers of genes in Real-time PCR assay
From: Genomic profiling of rectal adenoma and carcinoma by array-based comparative genomic hybridization
Gene | Forward primer | Backward primer | Size (bp) |
|---|---|---|---|
GAPDH | GGTCGTATTGGGCGCCTGGTC | TGACGGTGCCATGGAATTTGCCA | 148 |
KIFC1 | TCTCTGGGTGGTAGTGCTAAGA | TAAGTCACTTCCTGTTGGCCTG | 148 |
SOX4 | GACCGGGACCTGGATTTTAACT | TGAAAACCAGGTTGGAGATGCT | 133 |
PBX2 | AAGTTCCAAGAGGAGGCAAACA | TCCTGAGAGATTGAAAGAGCCG | 132 |
ESRRG | GCTATCCTGCAGCTGGTAAAGA | GCTATCCTGCAGCTGGTAAAGA | 133 |
KDM5B | CCCTCAGACACATCCTATTCCG | CAGTCCACCTCATCTCCTTCTG | 101 |
PTGS2 | TGTATCCTGCCCTTCTGGTAGA | AAGGAGAATGGTGCTCCAACTT | 85 |
EFNA1 | GTGGCAAAATCACTCACAGTCC | CTATGTAGAACCCGCACCTCTG | 91 |
BCL2 | AGGATTGTGGCCTTCTTTGAGT | CGGTTCAGGTACTCAGTCATCC | 113 |
SMAD4 | TGTTGATGACCTTCGTCGCTTA | ATGCTCTGTCTTGGGTAATCCG | 81 |
PHACTR3 | TATGACAGGAGGGCAGACAAAC | GCTTGCTTGATGCATGTACCTC | 118 |
C13orf27 | TCAGGCTCAGCAGATGAAATGT | TCCAGTGGATTTTATGGGGAGC | 85 |
PMEPA | CTGAGCCACTACAAGCTGTCTG | CTTCTGAGGACAGGGCATCTTC | 85 |
OXGR1 | ATCTTGAGGGTCATTCGGATCG | TGTCGCTGACCACCACATATAG | 148 |
GPNMB | GTCACTGTGATCTCCCTCTTGG | TTTGCACGGTTGAGAAAGACAC | 116 |