Skip to main content

Table 2 Characteristics of the samples.

From: Routine use of microarray-based gene expression profiling to identify patients with low cytogenetic risk acute myeloid leukemia: accurate results can be obtained even with suboptimal samples

Covariates

Overall

APLs

t(8;21)- AMLs

inv(16)-AMLs

NK-AMLs

Samples*

N = 206

N = 40

N = 39

N = 52

N = 57

Groups

     

Training Set AML samples

71

14

14

15

28

Training Set NBM&samples

18

--

--

--

--

Test Set AML samples*

111

24

23

35

29

Test Set AML cell lines

6

2

2

2

--

Samples with optimal QCC#

     

   Blast % < 60% (undiluted)

22

3

5

11

3

   Diluted at 50%

28

5

5

5

13

   Diluted at 75%

27

5

4

5

13

   Diluted at 90%

12

4

4

4

0

   Diluted at 95%

12

4

4

4

0

   Overall, blasts < 60%

101

21

22

29

29

   Overall, blasts ≥ 40% < 60%

23

2

2

6

13

   Overall, blasts ≥ 20% < 40%

40

6

9

10

15

   Overall, blasts ≥ 10% < 20%

15

4

4

6

1

   Overall, blasts ≥ 5% < 10%

14

5

4

5

0

   Overall, blasts < 5%

9

4

3

2

0

Samples with poor QCC#

10

3

1

6

0

   cRNA $ < 750 ng

7

1

1

5

0

   Low RIN%

2

2

0

0

0

   Low RIN + low cRNA

1

0

0

1

0

  1. * including the three AML cell lines. # quality control criteria. $ labeled cRNA to be hybridized on Illumina Beadchips.
  2. % RNA integrity number lower than 7.00. &NBM, normal bone marrow.