Skip to main content

Table 2 Characteristics of the samples.

From: Routine use of microarray-based gene expression profiling to identify patients with low cytogenetic risk acute myeloid leukemia: accurate results can be obtained even with suboptimal samples

Covariates Overall APLs t(8;21)- AMLs inv(16)-AMLs NK-AMLs
Samples* N = 206 N = 40 N = 39 N = 52 N = 57
Groups      
Training Set AML samples 71 14 14 15 28
Training Set NBM&samples 18 -- -- -- --
Test Set AML samples* 111 24 23 35 29
Test Set AML cell lines 6 2 2 2 --
Samples with optimal QCC#      
   Blast % < 60% (undiluted) 22 3 5 11 3
   Diluted at 50% 28 5 5 5 13
   Diluted at 75% 27 5 4 5 13
   Diluted at 90% 12 4 4 4 0
   Diluted at 95% 12 4 4 4 0
   Overall, blasts < 60% 101 21 22 29 29
   Overall, blasts ≥ 40% < 60% 23 2 2 6 13
   Overall, blasts ≥ 20% < 40% 40 6 9 10 15
   Overall, blasts ≥ 10% < 20% 15 4 4 6 1
   Overall, blasts ≥ 5% < 10% 14 5 4 5 0
   Overall, blasts < 5% 9 4 3 2 0
Samples with poor QCC# 10 3 1 6 0
   cRNA $ < 750 ng 7 1 1 5 0
   Low RIN% 2 2 0 0 0
   Low RIN + low cRNA 1 0 0 1 0
  1. * including the three AML cell lines. # quality control criteria. $ labeled cRNA to be hybridized on Illumina Beadchips.
  2. % RNA integrity number lower than 7.00. &NBM, normal bone marrow.