Characterization of isolated mitochondria from cells. A. Western blotting images of 6 marker proteins in the whole-cell protein samples (left column) and the isolated mitochondrial protein samples (right column). A total of 60 μg proteins was loaded and isolated by 12% SDS-PAGE, and transferred to PVDF membrane and incubated with primary antibodies (dilution 1:500) of each marker protein and second antibodies (dilution 1:2,000). B. Electron micrograph of isolated mitochondrial samples from cells (20,000 ×).