Figure 4

Networks of ‘cell death of tumor cell lines’ function and corresponding transcripts. Networks were constructed with IPA software using genes DE and involved in ‘cell death of tumor cell lines’ following CDV treatment of (A) SiHa, (B) HeLa, or (C) HaCaT cells. A network is a graphical representation of the molecular relationships between molecules (nodes). The biological relationship between two nodes is represented as an edge connecting two nodes. All edges are supported by information from the literature stored in the Ingenuity Pathways Knowledge Base. The intensity of the node color indicates the degree of up-regulation (red) or down-regulation (green) following CDV treatment. Canonical pathways identified by IPA in the networks are shown in blue. Except for DAB2, SERPINI1, CFH, and NEDD9 in SiHa; NEDD9, DKK1, DDX58, CDF15, DUSP4, CASP1, IGFBP3, F2R, SLC7A11, and ASNS in HeLa; XAF1, TRIM21, USP18, TNFSF13B, STAT4, GLRX, DDX58, GEM, TBX3, BMP2, TUBA1A, STAT1, CTGF, IL8, GDF15, CYP1B1, PTHLH, THBS1, CASP1, IL11, CLU, and CTSL2 in HaCaT; no changes in expression for genes included in the cell death networks built for the immortalized cells were observed in PHKs. Notably, BMP2 (SiHa); MYLK (HeLa); and NUPR1, NUF2, SPC25, BUB1, TOP2A, DEPC1, CCNB1, PBK, TGFB2, TP63, ABCG2, CCNA2, MKI67, SSPN, NRG1, MAP3K8, and ALOX5 (HaCaT) were inversely regulated in PHKs.