Skip to main content

Table 1 CDV metabolism and incorporation

From: Cidofovir selectivity is based on the different response of normal and cancer cells to DNA damage

  SiHa [pmol/10e6 cells] HeLa [pmol/10e6 cells] HaCaT [pmol/10e6 cells] PHKs [pmol/10e6 cells]
Methanol-soluble fraction     
CDV 4.55 ± 0.03 3.20 ± 0.25 1.76 ± 0.11 4.62 ± 0.63
CDVp 2.35 ± 0.13 1.61 ± 0.04 0.38 ± 0.08 0.78 ± 0.14
CDVpp 3.50 ± 0.02 2.93 ± 0.08 1.56 ± 0.01 3.56 ± 0.28
CDVp-choline 11.90 ± 0.37 8.73 ± 0.24 2.65 ± 0.12 9.18 ± 1.34
Sum of all metabolites 22.29 ± 0.23 16.47 ± 0.06 6.34 ± 0.08 18.13 ± 2.39
Digestion of methanol- insoluble fraction     
Incorporated CDV 18.39 ± 0.54 13.17 ± 1.43 8.62 ± 1.11 1.96 ± 0.71
  1. Cells were incubated for 72 h with 10 μCi radiolabeled CDV (final concentration of 50 μg/ml) to asses metabolism and incorporation in cellular DNA. Data are the mean ± SD of two experiments.