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Table 1 CDV metabolism and incorporation

From: Cidofovir selectivity is based on the different response of normal and cancer cells to DNA damage

 

SiHa [pmol/10e6 cells]

HeLa [pmol/10e6 cells]

HaCaT [pmol/10e6 cells]

PHKs [pmol/10e6 cells]

Methanol-soluble fraction

    

CDV

4.55 ± 0.03

3.20 ± 0.25

1.76 ± 0.11

4.62 ± 0.63

CDVp

2.35 ± 0.13

1.61 ± 0.04

0.38 ± 0.08

0.78 ± 0.14

CDVpp

3.50 ± 0.02

2.93 ± 0.08

1.56 ± 0.01

3.56 ± 0.28

CDVp-choline

11.90 ± 0.37

8.73 ± 0.24

2.65 ± 0.12

9.18 ± 1.34

Sum of all metabolites

22.29 ± 0.23

16.47 ± 0.06

6.34 ± 0.08

18.13 ± 2.39

Digestion of methanol- insoluble fraction

    

Incorporated CDV

18.39 ± 0.54

13.17 ± 1.43

8.62 ± 1.11

1.96 ± 0.71

  1. Cells were incubated for 72 h with 10 μCi radiolabeled CDV (final concentration of 50 μg/ml) to asses metabolism and incorporation in cellular DNA. Data are the mean ± SD of two experiments.