Association between E2-induced alternative splicing of FAS and FGFR2, and cellular response to apoptosis and proliferation, respectively. A) The effect of E2 on FAS-activation induced apoptosis. Cells were treated with 50 ng/ml FAS-activating antibody with or without co-treatment with E2 for 48 hours. Annexin V labeling followed by flow cytometry was used to measure apoptosis. Representative flow cytometry data from three experiments are shown. B) KGF partially overcomes the effect of tamoxifen on E2-induced cell proliferation. Cells in 96-well plate were treated with E2 (0.1 nM), 4-hydroxy tamoxifen (0.25 nM), KGF (20 ng/ml) or indicated combinations for 48 hours. BrDU-ELISA was used to measure the rate of cell proliferation. Results are from a representative of three experiments with six wells per treatment in each experiment. Reproducibly statistically significant results are described in the text.