Figure 2

Visualization of data in the Validation Cohort, showing differential expression, Volcano Plot, Principal Component Analysis and Hierarchical clustering of 100 most highly differentially expressed transcripts from the Discovery Cohort. A: Fold change comparison. Fold changes were sorted by value in the discovery cohort (red line). The x-axis represents the numbered probe sets. Fold change direction is identical and in similar range for all probe sets in both cohorts. B: Volcano plot showing the qFDR and the fold changes for the 110 probe sets in the validation cohort after ANOVA. The qFDR and fold change are comparable in both cohorts. C: PCA utilizing the 110 probe sets from the validation cohort. The two groups are clearly separated indicating that the expression patterns of the transcripts are comparable in both cohorts. D: Hierarchical clustering of Normal and Uremic samples from the validation cohort based on 110 probe sets from the discovery cohort showing clear separation of both subject sets.