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Table 1 Parameters calculated by Cosinor analysis (fitting a cosine curve with a 24-hour period to raw data) of clock gene expression levels after serum-shock induced synchronization in normal fibroblasts and fibroblasts of patients affected by Hunter syndrome before and after 24 hours of treatment with idursulfase

From: Circadian transcriptome analysis in human fibroblasts from Hunter syndrome and impact of iduronate-2-sulfatase treatment

Fibroblast of control subject 1 Fibroblast of control subject 2
  MESOR Amplitude Acrophase P   MESOR Amplitude Acrophase P
ARNTL 0.578 0.401 60° 42′ 0.048 ARNTL 0.872 0.095 130° 30′ 0.747
ARNTL2 0.753 0.186 131° 58′ 0.734 ARNTL2 0.802 0.041 72° 33′ 0.948
CLOCK 0.834 0.061 45° 00′ 0.749 CLOCK 0.642 0.096 11° 11′ 0.827
PER1 0.813 0.106 51° 40′ 0.592 PER1 0.621 0.108 336° 40′ 0.818
PER2 0.777 0.271 277° 45′ 0.234 PER2 0.772 0.030 164° 21′ 0.983
PER3 0.420 0.469 258° 57′ 0.136 PER3 0.645 0.185 241° 35′ 0.825
CRY1 0.747 0.184 324° 45′ 0.577 CRY1 0.704 0.091 319° 46′ 0.842
Fibroblast of Hunter syndrome patient 1 not treated Fibroblast of Hunter syndrome patient 1 treated with idursulfase
  MESOR Amplitude Acrophase P   MESOR Amplitude Acrophase P
ARNTL 0.709 0.190 139° 04′ 0.493 ARNTL 0.499 0.406 69° 11′ 0.444
ARNTL2 0.466 0.383 204° 48′ 0.280 ARNTL2 0.597 0.110 101° 49′ 0.928
CLOCK 0.600 0.218 232° 42′ 0.825 CLOCK 0.500 0.255 37° 52′ 0.492
PER1 0.813 0.207 261° 52′ 0.516 PER1 0.321 0.257 28° 38′ 0.677
PER2 0.600 0.282 315° 00′ 0.294 PER2 0.585 0.196 330° 15′ 0.509
PER3 0.553 0.193 268° 32′ 0.867 PER3 0.484 0.350 305° 17′ 0.390
CRY1 0.461 0.253 161° 40′ 0.643 CRY1 0.399 0.237 2° 15′ 0.593
Fibroblast of Hunter syndrome patient 2 not treated Fibroblast of Hunter syndrome patient 2 treated with idursulfase
  MESOR Amplitude Acrophase P   MESOR Amplitude Acrophase P
ARNTL 0.552 0.365 103° 12′ 0.298 ARNTL 0.462 0.407 83° 41′ 0.163
ARNTL2 0.680 0.310 114° 09′ 0.260 ARNTL2 0.660 0.219 96° 41′ 0.349
CLOCK 0.662 0.316 119° 17′ 0.078 CLOCK 0.723 0.235 64° 40′ 0.296
PER1 0.597 0.169 54° 28′ 0.713 PER1 0.576 0.323 70° 31′ 0.235
PER2 0.420 0.196 9° 22′ 0.708 PER2 0.535 0.229 307° 31′ 0.682
PER3 0.383 0.257 322° 27′ 0.664 PER3 0.449 0.308 305° 32′ 0.572
CRY1 0.585 0.502 116° 45′ 0.009 CRY1 0.694 0.302 13° 30′ 0.039
  1. Cosinor, least-squares fit of single component cosine to all data. MESOR, rhythm-adjusted overall 24 h mean; Amplitude, peak-trough range of cosine model ; Acrophase, peak of cosine in degrees.