Circadian transcriptome analysis in human fibroblasts from Hunter syndrome and impact of iduronate-2-sulfatase treatment

Mazzoccoli, Gianluigi; Tomanin, Rosella; Mazza, Tommaso; D’Avanzo, Francesca; Salvalaio, Marika; Rigon, Laura; Zanetti, Alessandra; Pazienza, Valerio; Francavilla, Massimo; Giuliani, Francesco; Vinciguerra, Manlio; Scarpa, Maurizio

doi:10.1186/1755-8794-6-37

Table 1 Parameters calculated by Cosinor analysis (fitting a cosine curve with a 24-hour period to raw data) of clock gene expression levels after serum-shock induced synchronization in normal fibroblasts and fibroblasts of patients affected by Hunter syndrome before and after 24 hours of treatment with idursulfase

From: Circadian transcriptome analysis in human fibroblasts from Hunter syndrome and impact of iduronate-2-sulfatase treatment

Fibroblast of control subject 1					Fibroblast of control subject 2
	MESOR	Amplitude	Acrophase	P		MESOR	Amplitude	Acrophase	P
ARNTL	0.578	0.401	60° 42′	0.048	ARNTL	0.872	0.095	130° 30′	0.747
ARNTL2	0.753	0.186	131° 58′	0.734	ARNTL2	0.802	0.041	72° 33′	0.948
CLOCK	0.834	0.061	45° 00′	0.749	CLOCK	0.642	0.096	11° 11′	0.827
PER1	0.813	0.106	51° 40′	0.592	PER1	0.621	0.108	336° 40′	0.818
PER2	0.777	0.271	277° 45′	0.234	PER2	0.772	0.030	164° 21′	0.983
PER3	0.420	0.469	258° 57′	0.136	PER3	0.645	0.185	241° 35′	0.825
CRY1	0.747	0.184	324° 45′	0.577	CRY1	0.704	0.091	319° 46′	0.842
Fibroblast of Hunter syndrome patient 1 not treated					Fibroblast of Hunter syndrome patient 1 treated with idursulfase
	MESOR	Amplitude	Acrophase	P		MESOR	Amplitude	Acrophase	P
ARNTL	0.709	0.190	139° 04′	0.493	ARNTL	0.499	0.406	69° 11′	0.444
ARNTL2	0.466	0.383	204° 48′	0.280	ARNTL2	0.597	0.110	101° 49′	0.928
CLOCK	0.600	0.218	232° 42′	0.825	CLOCK	0.500	0.255	37° 52′	0.492
PER1	0.813	0.207	261° 52′	0.516	PER1	0.321	0.257	28° 38′	0.677
PER2	0.600	0.282	315° 00′	0.294	PER2	0.585	0.196	330° 15′	0.509
PER3	0.553	0.193	268° 32′	0.867	PER3	0.484	0.350	305° 17′	0.390
CRY1	0.461	0.253	161° 40′	0.643	CRY1	0.399	0.237	2° 15′	0.593
Fibroblast of Hunter syndrome patient 2 not treated					Fibroblast of Hunter syndrome patient 2 treated with idursulfase
	MESOR	Amplitude	Acrophase	P		MESOR	Amplitude	Acrophase	P
ARNTL	0.552	0.365	103° 12′	0.298	ARNTL	0.462	0.407	83° 41′	0.163
ARNTL2	0.680	0.310	114° 09′	0.260	ARNTL2	0.660	0.219	96° 41′	0.349
CLOCK	0.662	0.316	119° 17′	0.078	CLOCK	0.723	0.235	64° 40′	0.296
PER1	0.597	0.169	54° 28′	0.713	PER1	0.576	0.323	70° 31′	0.235
PER2	0.420	0.196	9° 22′	0.708	PER2	0.535	0.229	307° 31′	0.682
PER3	0.383	0.257	322° 27′	0.664	PER3	0.449	0.308	305° 32′	0.572
CRY1	0.585	0.502	116° 45′	0.009	CRY1	0.694	0.302	13° 30′	0.039

Cosinor, least-squares fit of single component cosine to all data. MESOR, rhythm-adjusted overall 24 h mean; Amplitude, peak-trough range of cosine model ; Acrophase, peak of cosine in degrees.

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ISSN: 1755-8794

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