Figure 7

Histological analysis of liver slices treated with CsA and CPZ. PCLS were cultured for 24 and 48 hours in the presence of DMSO (control) (A and B), 40 μM CsA (C and D) or 20 μM CPZ (E and F). Histology of PCLS treated with CsA revealed ballooning of hepatocytes at the outer parts of slices after 24 and 48 hours (C and D respectively). Histology of CPZ treated slices cultured for 24 and 48 hours (E and F respectively) revealed a slight increase in number of cells containing pycnotic nuclei compared to control slices cultured equally long (A and B).