Biochemical analysis and specific histological staining of liver slices treated with CsA. Bile acid (BA) (A) and triglyceride (TG) (B) levels in PCLS cultured for 24 or 48 hours in the presence of either DMSO or 40 μM cyclosporin A (CsA) were not affected by any of the conditions, X- axis represents the tested conditions and Y-axis represents content of BA (A) or TG (B) in nmol per slice. Glycogen was assessed by PAS staining in PCLS cultured for 24 h in the presence of DMSO (C) or 40 μM CsA (D) demonstrating the absence of glycogen in ballooning hepatocytes. Arrows in (C) indicate the presence of glycogen in controls. Fouchet staining in PCLS cultured for 24 h in the presence of DMSO (E) or 40 μM CsA (F) demonstrating accumulation of several vacuoles in CsA treated slices (see arrows in F). All pictures were taken using 100-fold magnification.