Median methylation levels for HAND1 in TOF patients and controls. (A) The schematic represents the distribution of the CpG site and CpG island in the HAND1 gene; (B) HAND1_M1 median methylation level; (C) HAND1_M2 median methylation level; (D) HAND1_M3 median methylation level. All of the values represent the median with the interquartile range. TSS, Transcription Start Sites; red vertical line, CpG sites; red thick bars, CpG islands; M,MassARRAY amplicon; The region between arrow, target amplicon. *p < 0.05, **p < 0.01, ***p < 0.001 (Mann–Whitney test).