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Table 1 Relative mRNA expression levels of NKX2-5 and HAND1 in samples from controls and patients with TOF

From: DNA methylation status of NKX2-5, GATA4 and HAND1in patients with tetralogy of fallot

Gene

Control (RQ value (2-ΔΔCt) a: mean ± SD,n = 6)

TOF (RQ value (2-ΔΔCt): mean ± SD, n = 30)

Pvalueb

NKX2-5

9.76 ± 7.39c

1.48 ± 3.45

0.0028

HAND1

7.55 ± 5.62

1.20 ± 0.41

0.0032

  1. a The RQ values (2-ΔΔCt) for control 1 was set as 1 and used for normalization for all samples; b Mann–Whitney test was performed; c “±” represents the meaning of addition and subtraction. One male, aged 1.9 years, was not included.