Chromosomal and gene aberrations in 20 human osteosarcoma samples and the status of RUNX2 gene copy number aberrations (CNAs). A. The recurrence pattern of CNAs in 20 human OS samples are illustrated in 2 microarray-based comparative genomic hybridization (aCGH) datasets (GSE19180and GSE9654). The x-axis indicates chromosome numbers and the y-axis indicates the aberration frequency of gains (positive)and losses (negative) for each measured aCGH probe, arranged based on their genomic coordinates along the x-axis. Dashed lines indicate the thresholds for significant recurrent aberrations. Measured sequences with aberration frequency that exceeded the thresholds are color-coded to emphasize the locations of significantly recurrent aberrations (red indicates significantly recurrent amplification; green, significantly recurrent deletion; grey, non-significant recurrence of aberrations). B. RUNX2 gene copy number aberrations in OS. Sample IDs of the 20 OS samples in aCGH datasets GSE9654 and GSE19180 are indicated on the bottom. OS1–19 represent the case IDs in GSE9654 and S6272–6285 represent the case IDs in GSE19180. Scatters denote copy number change of the RUNX2 gene. Lines in black and grey color denote the regional copy number value estimated by the circular binary segmentation (CBS) algorithm. Black lines denote significant amplification or deletion, whereas grey lines denote non-significant amplification or deletion. Twelve samples show amplification of the RUNX2 gene.