Correlation of WWOX, RUNX2 and VEGFA protein expression in human osteosarcoma

BMC Medical Genomics

Table 2 Correlation between gene copy number aberration and protein expression of WWOX, RUNX2 and VEGFA

Protein expression*	*WWOX*deletion (detected by aCGH in 10 samples, GSE 19180)		Pvalue	*RUNX2*amplification (detected by aCGH in 10 samples, GSE 19180)		Pvalue
	No (%)	Yes (%)		No(%)	Yes(%)
Negative	2 (40.0)	3 (100)	0.237	3 (50.0)	3 (100)	0.325
Weak positive	1 (20.0)	0 (0)		0 (0)	0 (0)
Moderate positive	0 (0)	0 (0)		2 (33.3)	0 (0)
Strong positive	2 (40.0)	0 (0)		1 (16.7)	0 (0)
VEGFA protein expression (detected by IHC in 54 cases)	Increased VEGFA gene copy number (detected by FISH in 54 cases)**			χ²	P value
VEGFA protein expression (detected by IHC in 54 cases)	No (%)	Yes (%)		χ²	P value
Negative	7 (43.8)	2 (6.5)		9.639	0.022***
Weak positive	5 (31.2)	15 (48.4)
Moderate positive	1 (6.2)	5 (16.1)
Strong positive	3 (18.8)	9 (29.0)

*IHC assay of RUNX2 protein expression was performed in nine cases from which fresh samples were used in the aCGH analysis. One aCGH data case was excluded from RUNX2 IHC analysis because of insufficient sample. For WWOX protein expression, only eight cases were used in the IHC assay.
**FISH results were unavailable for seven samples because the samples were insufficient and because of loss of tissues during the pretreatment process.
* **Significantly different P < 0.05.

ISSN: 1755-8794