Table 4 Primers for sequenom analysis of CpG methylation of the Slc2a4 promoter region
Amplicon ID | mus_Slc2a4_pp27 | mus_Slc2a4_pp46 |
|---|---|---|
Genomic co-ordinates | chr11:69761390-69761763 | chr11:69762346-69762668 |
Strand | – | – |
Length | 374 | 323 |
Sequence | CCTGGCCAATGGGTGTT | Â |
GTGAAGGGCGTGTCCTA | GGGAAGGGTTAATAGAA | |
TGGCGGGGCGGGAGTGG | GAGAGCCACCCCAGAA | |
GGAGGTGGCTTCAGCTCT | GTTAGCTACCCTGGTGC | |
CCGCATCTTTCCCCCTCA | AATCCACTAAGGTTCCT | |
AGCGGGTCTCACTAGAT | CGCTCTCCCTCTAGGTG | |
CCCGGAGAGCCTTGGTG | GCGCCAGAAGCCTTGC | |
CTCTCCGGTTCCGTGGGT | ACTTCTCTTGGGCCTTTT | |
TGTGGCAGTGAGTCCCAC | TCTGAATTGAGCTCTCTC | |
CAGACCCGCCCTTTGCAC | TCCACATTCTTCGCCAGC | |
ACGGCTTCCGAACGCCGG | TCTCTCCCTGGACGTGCTT | |
GGTCTCGTGCCGGCCAG | AGGTCGTGCCCTCTCAGCT | |
GCCCGGACCCTATACCC | GTAGACCCAAAACAGTAG | |
TATTCATTTTTTTCTTAT | CTGACTCTGGAAAGCTTGT | |
TGCAGCGCCTGAGTCTT | CGCCCACGCGGCCAGCACA | |
TTCTTCTTTTAAAACAAG | TGCCTGGAGGCTCAGGGA | |
ATGCCGTCGGGTTTCCAG | CTTCAGGGAGGGTGGTGT | |
CAGATCGGCTCTGACGTAA | GACTGGCGTGAGCACCTG | |
GGTTCAGCATACCGGGGC | TCCCTTGGGTCCCCTCCAAGA | |
GAATTGGGAAATCTGGTC | Â | |
CAGTTTTTCTTGGGCTGAGTT | ||
Left primer length | 23 | 27 |
Left primer sequence | aggaagagagTTTGGTTAAT | aggaagagagGGGAAGGGTTA |
GGGTGTTGTGAAG | ATAGAAGAGAGTTATT | |
Right primer length | 25 | 25 |
Right primer sequence | cagtaatacgactcactatagggagaag | cagtaatacgactcactatagggagaagg |
gctAACTCAACCCAAAAAA | ctTCTTAAAAAAAACCCAA | |
| Â | AACTAAACC | AAAACAAA |