Table 1 Sequences of primers according to experiment

Bisulfite direct sequencing

EP6

Forward: 5′-GAT GCG TTA GAT TTA AGG GAG G-3′

Reverse: 5′-CTC ACT CTC ACG AAA CCC CTC-3′

EP7

Forward: 5′-GAG ATG TTT AGC GTT TGT GGG-3′

Reverse: 5′-AAC TAA TTA CAT AAA ACC CAC CC-3′.

qPCR

EP6

Forward: 5′-TGA ATC AGT TCA CCG ACA GC-3′

Reverse: 5′-GAA ACA ACC TGG CCA TTC TC-3′

EP7

Forward: 5′-CCG TTA TAT GGA TGC CTT GG-3′

Reverse: 5′-AAA CTG TTG GGC TGA ACT GC-3′

EP9

Forward: 5′-GAC CCA GA CGA TAC CTG GAA-3′

Reverse: 5′- CTG AAC AAA ACT CGG CTT C-3′