Table 3 Low RNA-yield clinical specimens: Nanodrop RNA 260/280 ratio, aRNA concentration and GeneChip QC metrics
Case number | Specimen type | Post-sort no. of CD138+ cells | RNA conc. (ng/μL) | aRNA conc. (ng/μL) | Chip QC Metrics (Fail, Warning, Pass) | Pass/Fail Chip QC |
|---|---|---|---|---|---|---|
RE13-000082 | Archival samples with low yield | 36,000 | 11.0 | 1506.1 | 0,1,6 | Pass |
RE13-000079 | Archival samples with low yield | 32,000 | 5.3 | 708.6 | 0,1,6 | Pass |
RE13-000080 | Archival samples with low yield | 1,896,000 | 6.4 | 689.7 | 0,1,6 | Pass |
RE13-000076 | Archival samples with low yield | 40,000 | 3.1 | 545.1 | 0,1,6 | Pass |
RE13-000092 | Fresh sample with low yield | 22,000 | 10.4 | 533 | 0,0,7 | Pass |
RE13-000074 | Archival samples with low yield | 80,000 | 10.8 | 455.7 | 0,1,6 | Pass |
RE13-000063 | Archival samples with low yield | 76,000 | 8.1 | 426.4 | 0,0,7 | Pass |
RE13-000077 | Archival samples with low yield | 90,000 | 6.1 | 410.1 | 0,1,6 | Pass |
RE13-000089 | Fresh sample with low yield | 32,000 | 9.1 | 383.3 | 0,0,7 | Pass |
RE13-000064 | Archival samples with low yield | 180,000 | 5.5 | 339.9 | 0,1,6 | Pass |
RE13-000073 | Archival samples with low yield | 43,000 | 10.4 | 311.1 | 0,0,7 | Pass |
RE13-000075 | Archival samples with low yield | 80,000 | 2.4 | 304 | 0,1,6 | Pass |
RE13-000066 | Archival samples with low yield | 18,000 | 6.9 | 290.4 | 0,1,6 | Pass |
RE13-000085 | Fresh sample with low yield | 23,000 | 13.3 | 286 | 0,1,6 | Pass |
RE13-000069 | Archival samples with low yield | 24,000 | 8.3 | 281.1 | 0,1,6 | Pass |
RE13-000088 | Fresh sample with low yield | 20,000 | 9.6 | 227.8 | 0,1,6 | Pass |
RE13-000081 | Archival samples with low yield | 692,000 | 8.2 | 361.7 | 3,2,2 | Fail |
RE13-000084 | Fresh sample with low yield | 36,000 | 5.8 | 341.6 | 1,0,6 | Fail |
RE13-000067 | Archival samples with low yield | 188,000 | 6.2 | 271.2 | 0,3,4 | Fail |
RE13-000068 | Archival samples with low yield | 470,000 | 7.7 | 263.3 | 2,1,4 | Fail |
RE13-000071 | Archival samples with low yield | 20,000 | 7.7 | 254.6 | 0,3,4 | Fail |
RE13-000061 | Archival samples with low yield | 60,000 | 3.1 | 220 | 0,3,4 | Fail |
RE13-000083 | Archival samples with low yield | 192,000 | 5.4 | 215.5 | 2,4,1 | Fail |
RE13-000078 | Archival samples with low yield | 17,000 | 3.4 | 214.6 | 1,2,4 | Fail |
RE13-000065 | Archival samples with low yield | 36,000 | 5.5 | 208.4 | 1,2,4 | Fail |
RE13-000090 | Fresh sample with low yield | 18,000 | 4.8 | 166.6 | 0,3,4 | Fail |
RE13-000091 | Fresh sample with low yield | 9,000 | 6.2 | 144.7 | 0,3,4 | Fail |
RE13-000062 | Archival samples with low yield | 10,000 | 2.4 | 122.8 | 2,3,2 | Fail |
RE13-000070 | Archival samples with low yield | 30,000 | 2.5 | 100.5 | 3,2,2 | Fail |
RE13-000086 | Fresh sample with low yield | 5,000 | 0.9 | 94.5 | 1,5,1 | Fail |