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Table 1 Primers used for confirmation by qRT-PCR of the IL1B, RGS1, EGR2, FOXO3A, SOD2 and HIF1A genes

From: Integrative analysis of the transcriptome profiles observed in type 1, type 2 and gestational diabetes mellitus reveals the role of inflammation

Gene Accesion numbers Primer forward Primer reverse Size (bp)
IL1B NM_000576.2 5′-CCACAGACCTTCCAGGAGAA-3′ 5′-GTGATCGTACAGGTGCATCG-3′ 121
RGS1 NM_002922.3 5′-TGGCTGGCTTGTGAAGACTA-3′ 5′-GATTCTCGAGTGCGGAAGTC-3′ 131
EGR2 NM_000399.3 5′-GGTGACCATCTTTCCCAATG-3′ 5′-TATGGGAGATCCAACGACCT-3′ 120
FOXO3A NM_001455.3 5′-GTGCTAAGCAGGCCTCATCT-3′ 5′-TTGGCAAAGGGTTTTCTCTG-3′ 119
SOD2 NM_001024465.1 5′-GACAAACCTCAGCCCTAACG-3′ 5′-TTGGACACCAACAGATGCAG-3′ 124
HIF1A NM_181054.2 5′-TCAGCTATTTGCGTGTGAGG-3′ 5′-AAAACCATCCAAGGCTTTCA-3′ 107
GAPDH NM_002046.3 5′-CTCTGCTCCTCCTGTTCGAC-3′ 5′-ACGACCAAATCCGTTGACTC-3′ 112
HPRT1 NM_000194.2 5′-GACCAGTCAACAGGGGACAT-3′ 5′-CTGCATTGTTTTGCCAGTGT-3′ 111
  1. GAPDH and HPRT1 were selected as endogens.