Table 1 Primers used for confirmation by qRT-PCR of the IL1B, RGS1, EGR2, FOXO3A, SOD2 and HIF1A genes
Gene | Accesion numbers | Primer forward | Primer reverse | Size (bp) |
|---|---|---|---|---|
IL1B | NM_000576.2 | 5′-CCACAGACCTTCCAGGAGAA-3′ | 5′-GTGATCGTACAGGTGCATCG-3′ | 121 |
RGS1 | NM_002922.3 | 5′-TGGCTGGCTTGTGAAGACTA-3′ | 5′-GATTCTCGAGTGCGGAAGTC-3′ | 131 |
EGR2 | NM_000399.3 | 5′-GGTGACCATCTTTCCCAATG-3′ | 5′-TATGGGAGATCCAACGACCT-3′ | 120 |
FOXO3A | NM_001455.3 | 5′-GTGCTAAGCAGGCCTCATCT-3′ | 5′-TTGGCAAAGGGTTTTCTCTG-3′ | 119 |
SOD2 | NM_001024465.1 | 5′-GACAAACCTCAGCCCTAACG-3′ | 5′-TTGGACACCAACAGATGCAG-3′ | 124 |
HIF1A | NM_181054.2 | 5′-TCAGCTATTTGCGTGTGAGG-3′ | 5′-AAAACCATCCAAGGCTTTCA-3′ | 107 |
GAPDH | NM_002046.3 | 5′-CTCTGCTCCTCCTGTTCGAC-3′ | 5′-ACGACCAAATCCGTTGACTC-3′ | 112 |
HPRT1 | NM_000194.2 | 5′-GACCAGTCAACAGGGGACAT-3′ | 5′-CTGCATTGTTTTGCCAGTGT-3′ | 111 |